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Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of homologous strain 25238 in mice

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of homologous strain 25238 in mice

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques: Enzyme-linked Immunosorbent Assay

Anti-LOS AFCs elicited in different tissues of mice after dLOS-CRM conjugate immunization

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Anti-LOS AFCs elicited in different tissues of mice after dLOS-CRM conjugate immunization

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques:

Time course of bacterial recovery from mouse lungs challenged with M. catarrhalis strain 25238 after i.n. immunization with dLOS-CRM. Mice received i.n.-administered 10-μl aliquots of PBS containing a mixture of 5 μg of dLOS-CRM and 1 μg of CT four times at 1-week intervals or were kept as naïve mouse controls. One week after the last immunization, all the mice were challenged (via aerosol) with 10 ml of M. catarrhalis strain 25238 (5 × 108 CFU/ml) in a nebulizer. Lungs were collected, homogenized, and cultured for bacterial counts at 0, 3, 6, 12, 24, and 48 h postchallenge. Bacterial recoveries from lungs are expressed as geometric means ± SD (error bars) from eight mice. **, P < 0.01 (for comparison with naïve mouse controls).

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Time course of bacterial recovery from mouse lungs challenged with M. catarrhalis strain 25238 after i.n. immunization with dLOS-CRM. Mice received i.n.-administered 10-μl aliquots of PBS containing a mixture of 5 μg of dLOS-CRM and 1 μg of CT four times at 1-week intervals or were kept as naïve mouse controls. One week after the last immunization, all the mice were challenged (via aerosol) with 10 ml of M. catarrhalis strain 25238 (5 × 108 CFU/ml) in a nebulizer. Lungs were collected, homogenized, and cultured for bacterial counts at 0, 3, 6, 12, 24, and 48 h postchallenge. Bacterial recoveries from lungs are expressed as geometric means ± SD (error bars) from eight mice. **, P < 0.01 (for comparison with naïve mouse controls).

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques: Cell Culture

Comparison of protective effect induced by different doses of dLOS-CRM conjugate in mouse lungs challenged with the M. catarrhalis strain 25238. Mice were i.n. immunized at 1-week intervals with one to five doses consisting of 10 μl of PBS containing a mixture of 5 μg of dLOS-CRM and 1 μg of CT or were kept as naïve mouse controls. One week after last immunization, all the mice were challenged (via aerosol) with 10 ml of M. catarrhalis strain 25238 (5 × 108 CFU/ml) in a nebulizer, and their lungs were collected 6 h postchallenge. Bacterial recoveries from the lungs are expressed as geometric means + SD (error bars) from eight mice. Statistical significance for comparison with naïve mouse controls: **, P < 0.01; *, P < 0.05.

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Comparison of protective effect induced by different doses of dLOS-CRM conjugate in mouse lungs challenged with the M. catarrhalis strain 25238. Mice were i.n. immunized at 1-week intervals with one to five doses consisting of 10 μl of PBS containing a mixture of 5 μg of dLOS-CRM and 1 μg of CT or were kept as naïve mouse controls. One week after last immunization, all the mice were challenged (via aerosol) with 10 ml of M. catarrhalis strain 25238 (5 × 108 CFU/ml) in a nebulizer, and their lungs were collected 6 h postchallenge. Bacterial recoveries from the lungs are expressed as geometric means + SD (error bars) from eight mice. Statistical significance for comparison with naïve mouse controls: **, P < 0.01; *, P < 0.05.

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques:

Dose pattern of LOS antibody responses elicited after i.n. immunization with dLOS-CRM conjugate

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Dose pattern of LOS antibody responses elicited after i.n. immunization with dLOS-CRM conjugate

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques: Enzyme-linked Immunosorbent Assay

Effects of different immunizations routes with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance in mice

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Effects of different immunizations routes with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance in mice

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques: Enzyme-linked Immunosorbent Assay

Anti-LOS AFCs elicited in different tissues of mice by dLOS-CRM conjugate

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Anti-LOS AFCs elicited in different tissues of mice by dLOS-CRM conjugate

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques:

Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain O35E (serotype A) in mice

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain O35E (serotype A) in mice

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques:

Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain 26397 (serotype B) in mice a

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain 26397 (serotype B) in mice a

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques:

Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain 26404 (serotype C) in mice

Journal:

Article Title: Specific Immune Responses and Enhancement of Murine Pulmonary Clearance of Moraxella catarrhalis by Intranasal Immunization with a Detoxified Lipooligosaccharide Conjugate Vaccine

doi: 10.1128/IAI.70.11.5982-5989.2002

Figure Lengend Snippet: Effect of i.n. immunization with dLOS-CRM conjugate on LOS antibody responses and bacterial clearance of the heterologous strain 26404 (serotype C) in mice

Article Snippet: The amount of vaccine preparations inhaled was formulated to contain 5 μg of dLOS-CRM (carbohydrate content) mixed with 1 μg of cholera toxoid (CT) (List Biological Laboratories, Inc, Campbell, Calif.) as an adjuvant.

Techniques: